About iotaSciences Single-Cell Solutions

iotaSciences offers cutting-edge automated solutions for gentle single-cell isolation and single-cell cloning, revolutionizing cell biology research worldwide. Our proprietary GRID technology, licensed from Oxford University (doi:10.1073/pnas.1805449115), utilizes inert liquids to create miniature cell culture chambers on standard 6 cm culture dishes. Each chamber provides an ultra-low volume environment of approximately 200 nanolitres, maintaining high concentrations of autocrine factors essential for single-cell survival while reducing reagent consumption by over 10-fold compared to traditional 96-well plate methods.

The iotaSciences product portfolio includes three integrated platforms: isoPick for gentle automated cell dispensing and picking, isoCell for comprehensive single-cell culture with automated liquid handling, and isoHub for monoclonality verification and clone tracking. The optional Image Capture System provides complete documentation of single-cell clonal outgrowth, meeting stringent regulatory requirements for monoclonal antibody production and cell line development.

Our technology excels in applications requiring the highest level of cell viability, including CRISPR-Cas9 gene editing, iPSC (induced pluripotent stem cell) engineering, antibody discovery, single-cell omics, and therapeutic cell line development. The ultra-gentle handling approach employs low pressure and low shear forces, making it ideal for sensitive cells such as iPSCs, stem cells, and primary cells. With optional temperature control and heating capabilities, cells maintain optimal metabolic activity throughout the entire workflow.

The GRID technology eliminates optical edge effects typically associated with conventional culture plasticware, enabling complete visualization of every chamber under standard microscopy. Each 6 cm plate contains 256 individual chambers with an area of only 3.2 mm² each, allowing high-throughput processing while maintaining single-cell resolution. The isoHub microscope seamlessly integrates with isoPick or isoCell systems via Bluetooth wireless data transfer, enabling efficient workflow automation and comprehensive clone tracking across multiple plates.

iotaSciences provides expert cell line engineering services specialized in human iPSC CRISPR-Cas9 gene editing. Our bespoke genome engineering services deliver high-quality, precisely edited iPSC cell models, allowing researchers to focus on scientific discoveries rather than technical challenges. The nanolitre-scale culture environment significantly improves cloning efficiency for precious edited cells, reducing experimental time and costs.

For antibody discovery and monoclonal antibody production, iotaSciences offers unambiguous proof of monoclonality through direct visualization and automated imaging documentation. This comprehensive documentation satisfies regulatory requirements from agencies such as FDA and EMA, streamlining the path to therapeutic antibody development and commercialization. The system supports various downstream applications including 96-well plates, PCR tubes, and mass spectrometry vials.

The isoCell system represents the most compact automated single-cell culture platform available, fitting seamlessly into standard biosafety cabinets without obstructing ongoing cell culture work. Its intuitive user interface and knowledge-driven workflows reduce operator variability and increase experimental reproducibility. With precise control down to 200 nanolitres of liquid handling, the automated arm mimics gentle manual pipetting while eliminating human error and fatigue.

Single-cell omics applications benefit from iotaSciences' gentle isolation technology, which preserves cell integrity prior to genomics, transcriptomics, or proteomics analysis. The ultra-low volume culture environment is particularly advantageous for rare cell studies and heterogeneity analysis, where every cell counts. Researchers can confidently sort and culture single cells with minimal stress, ensuring high-quality downstream sequencing and analysis results.

Trusted by leading academic institutions and biopharmaceutical companies globally, iotaSciences technology accelerates research timelines and improves success rates across diverse cell biology applications. The modular system design allows laboratories to start with essential components and expand capabilities as needs evolve. Comprehensive technical support, training, and application development assistance ensure optimal implementation and ongoing success.

Whether developing CAR-T cell therapies, establishing stable cell lines, screening hybridomas, or advancing regenerative medicine research, iotaSciences provides the precision, gentleness, and automation required to get the most out of every cell. Our commitment to innovation and quality has made iotaSciences the preferred choice for researchers pushing the boundaries of single-cell technology and advancing the therapies of tomorrow.