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Leprechaun Virus and Exosome Analysis System - Advanced Titer and Phenotyping Platform

The Leprechaun system from Unchained Labs represents a breakthrough in virus vector and exosome characterization, offering comprehensive biophysical analysis without the need for extensive sample purification. This innovative platform combines interferometry-based sizing with immunofluorescence detection to deliver precise measurements of lentivirus titer, particle structure, RNA content, and exosome phenotype in a single, high-throughput workflow.

Designed for gene therapy development, biologics manufacturing, and extracellular vesicle research, Leprechaun provides triple verification of lentiviral particles by confirming size, capsid structure, and RNA content simultaneously. The system can analyze up to 16 samples per run using the Luni consumable, requiring only 1-25 µL of sample volume. This minimal sample requirement makes it ideal for precious early-stage materials and enables comprehensive quality control throughout the entire production process.

The platform's unique capability to analyze crude samples directly eliminates time-consuming purification steps while delivering accurate titer measurements ranging from 1×10⁷ to 5×10⁸ viral particles/mL for lentivirus. For exosomes, the system detects concentrations from 5×10⁶ to 5×10⁸ particles/mL with 1-hour incubation, or as low as 5×10⁵ particles/mL with extended 16-hour incubation protocols.

Leprechaun's advanced interferometry technology enables single-particle sizing down to 35 nm with ≤5% accuracy, covering the full range of exosomes, lentiviral vectors, and even smaller exomeres. The integrated fluorescence microscopy system utilizes three LED channels (470 nm blue, 567 nm green, 623 nm red) for multi-color immunofluorescence analysis, allowing simultaneous detection of up to four different protein markers with ≤5% fluorescence intensity precision.

The Lentivirus EV Contaminant Kit enables comprehensive purity assessment by distinguishing between intact lentiviral particles, empty capsids, extracellular vesicle contaminants, and soluble p24 protein. This capability ensures product quality and helps optimize purification strategies throughout the manufacturing workflow. Researchers can monitor titer, structure, and purity at every process step, from harvest through final formulation.

For exosome research, Leprechaun delivers concentration and phenotype data from diverse sample types including cell culture media, plasma, serum, cerebrospinal fluid, and urine. The pan-tetraspanin capture approach combined with specific markers like CD9, CD63, and CD81 provides detailed characterization of extracellular vesicle populations. The Flex Kit option allows customization with user-provided antibodies for unique applications.

Leprechaun Analysis software automates data processing with intelligent outlier removal and background subtraction, delivering instant results for both lentivirus and exosome samples. Customizable size ranges and fluorescent thresholds provide flexibility while maintaining analytical rigor. The compact instrument (23.4 × 47.8 × 35.2 cm, 20.5 kg) includes a dedicated Windows 11 computer and operates on standard laboratory power (110-220V AC).

This comprehensive platform accelerates gene therapy development, streamlines biologics QC workflows, and enables breakthrough discoveries in extracellular vesicle biology. By eliminating the need for purification while maintaining analytical precision, Leprechaun reduces time-to-results from days to hours and provides actionable data for critical process decisions at every manufacturing stage.