服務專線：0800.898.178 info@jnhtech.com.tw
完全解放雙手的緩衝液交換方案
Unagi 全自動化系統讓您不再被綁在實驗台前，一鍵啟動後即可處理其他工作
小型化的緩衝液交換解決方案
緩衝液交換與樣品濃縮是實驗前處理中最耗時且需要人力看守的步驟。過程冗長、需要持續在旁監控，而且往往在以為完成時還需要再次調整。Unagi 是首款完全免手動操作的桌上型緩衝液交換系統，專為將這些繁瑣工作從您的待辦清單中移除而設計，讓您可以專注於其他更重要的研究工作。
適用樣品類型
Protein（蛋白質）
mRNA & DNA（核酸）
AAV（腺相關病毒載體）
LNP（脂質奈米顆粒）
Unagi 桌上型 UF/DF 自動化系統
平行處理彈性體積
單次可平行處理多達 8 個樣品，每個樣品體積範圍從 0.5 到 8 mL，提供從早期研發到大量製備所需的通量與體積彈性。
高回收率
在緩衝液交換或脫鹽過程中達到 ≥96% 的樣品回收率，確保珍貴的蛋白質、核酸、病毒載體與 LNP 樣品不浪費。
定義體積濃縮
可將樣品從 48 mL 濃縮至 8 mL，具備精確的自動化最終體積控制功能。
耐用低吸附耗材
提供再生纖維素膜（10、30、100 kDa MWCO）與聚醚碸膜（30、100 kDa MWCO）選擇，防止樣品損失與吸附，確保乾淨分離。
完全免手動操作
裝載樣品後即可讓 Unagi 自動執行整個 UF/DF 循環，無需監控離心柱、過濾步驟或離心機循環，完全解放您的雙手。
智慧化流程控制
採用超音波感測技術即時監控體積變化，自動調整壓力與混合參數，確保每個樣品都達到目標交換率與濃度。
客製化的耗材配置
只需將 1 到 8 個樣品分別加入 Una 耗材中，旋轉固定於托盤上即可準備就緒。每個 Una 可容納 0.5 到 8 mL 的樣品。系統提供再生纖維素膜（10、30、100 kDa MWCO）與聚醚碸膜（30、100 kDa MWCO）兩種選擇，確保您的樣品保持完整、不被吸附，液體順利通過濾膜，達到 ≥96% 的回收率。
Una 耗材規格
| 膜材質 | MWCO 選項 | 樣品容量 |
|---|---|---|
| Regenerated Cellulose（再生纖維素） | 10, 30, 100 kDa | 0.5-8 mL |
| Polyethersulfone（聚醚碸） | 30, 100 kDa | 0.5-8 mL |
關鍵優勢
- 低蛋白質吸附特性，最大化樣品回收
- 多種 MWCO 選擇，適配不同分子量需求
- 一次性使用設計，避免交叉污染
- 簡易旋轉固定機制，快速裝載
精密的技術配方
Unagi 將 UF/DF（Ultrafiltration/Diafiltration，超濾/透析濾）緩衝液交換技術發揮到極致。系統首先使用超音波感測器測量初始樣品體積，精確掌握起始條件。接著對每個 Una 施加壓力並溫和混合樣品以促進液體流動。系統會再次測量體積並以新緩衝液填充每個 Una。此過程持續重複直到 Unagi 達到您設定的目標交換百分比與體積。
1
體積測量
超音波感測器精確測量初始樣品體積
2
壓力 + 混合
施加可程式化壓力（15/30/60 psi）並溫和混合促進過濾
3
液體過濾
小分子通過濾膜，大分子保留於 Una 中
4
體積確認
再次測量體積確保過濾進度
5
緩衝液補充
自動填充新緩衝液至原始體積
6
循環完成
達到目標交換率後可選擇性濃縮
簡易的操作流程
Step 1
設定實驗參數
Unagi 操作極為簡單，任何人都能輕鬆上手。首先選擇您要執行的實驗類型，接著輸入要交換的分子種類、樣品量以及目標緩衝液。最後輸入交換百分比與最終體積（如需濃縮樣品）。
Step 2
裝載耗材與試劑
現在只需依照 Unagi 的逐步指示操作。將裝有樣品的 Una 托盤放置於前方，在左側放入裝有緩衝液的 Falcon 管，右側放入吸頭盒，Unagi 即準備就緒。沒開玩笑，就是這麼簡單。
裝載位置說明
- 前方：Una 樣品托盤（1-8 個樣品）
- 左側：緩衝液 Falcon 管
- 右側：1000 µL 吸頭盒
Step 3
啟動並監控進度
按下啟動鍵後讓 Unagi 開始工作。此時您可以離開去處理其他事務，但也歡迎隨時回來查看。系統會追蹤已完成的交換循環次數、提供當前過濾循環資訊，並顯示每個樣品的交換百分比。如果趕時間，您可以暫停實驗取出已完成的樣品，或讓其繼續運行。無論如何，Unagi 都會在完成時通知您。
即時監控功能
- 交換循環進度追蹤
- 當前過濾循環狀態
- 每個樣品的交換百分比顯示
- 可暫停/繼續實驗執行
- 預估剩餘時間顯示
樣品濃縮功能
某些樣品過於稀釋需要特殊處理。Unagi 能將這些樣品濃縮至可用範圍。選擇「Reduce Sample Volume」應用程式，Unagi 可在單次運行中將多達 8 個稀釋樣品從 48 mL 濃縮至 8 mL。之後您可以接續進行緩衝液交換實驗或進一步濃縮。
濃縮規格
| 起始體積範圍 | 最高 48 mL（使用 Falcon 管） |
| 最終體積範圍 | 8 mL（轉移至 Una） |
| 平行處理數 | 最多 8 個樣品 |
| 濃縮倍數 | 最高 6 倍濃縮 |
| 樣品回收率 | ≥96% |
濃縮應用場景
- 培養上清液濃縮
- 透析後樣品濃縮
- 純化步驟間濃縮
- 下游分析前樣品準備
完整的實驗記錄
Unagi 追蹤並記錄實驗過程中的每一個動作與事件。系統會產生詳細報告，包含最終體積與濃度、每個循環的流速以及每個樣品的最終交換百分比。這些報告可輕鬆放入實驗室記錄本、上傳至 LIMS 系統，或是貼在您的冰箱上。
Final Formulations
記錄每個樣品的最終體積、濃度、測量起始體積、最終體積、交換百分比及濃縮倍數
Experiment Cycles
詳列緩衝液交換循環次數與濃縮循環次數的完整記錄
Experiment Parameters
保存混合速度、混合循環時間、壓力設定等所有實驗參數
Export Options
提供 Experiment Log、Summary Report、Raw Report 等多種匯出格式，支援列印與數位保存
法規遵循與數據完整性
所有實驗數據均包含時間戳記、儀器 ID 與實驗描述，符合實驗室數據管理與品質控制要求。報告可直接整合至電子實驗室記錄本（ELN）或實驗室資訊管理系統（LIMS）。
技術規格
Application
| Buffer exchange volume range | 0.5-8 mL |
| Formulations | Up to 8 formulations in parallel |
| Sample types | Antibodies and other proteins, nucleic acids, and AAVs |
| Exchange time at full volume (96%, at 10 mg/mL IgG) |
4.5 hours* |
| Protein concentration (range) | Up to 200 mg/mL* |
| Target concentration accuracy | ±10%* |
| Sample recovery | ≥96%* |
System
| Volume measurement | Ultrasonic sensor |
| Exchange pressures | 15, 30 or 60 psi |
| Operating temperature | Room temperature |
| Buffer exchange orbital mixing | Optimized at 700 rpm Duty cycle programmable |
| Physical | 63 cm W x 57 cm D x 66 cm H; 65 kg |
| Electrical | Voltage 100-260 VAC, 50-60 Hz |
| Nitrogen or CDA requirement | Pressure 0.55–0.69 MPa (80–100 psi) Flow rate 0.25 L/s (0.5 cfm) minimum |
Consumables
| Una | 10, 30, and 100 kDa, regenerated cellulose 30 and 100 kDa polyethersulfone |
| Disposable tips | 1000 µL non-filtered, automatic re-use up to 12 times per exchange |
| Dispense precision | 0.3-8 mL: ≤1% |
*Sample and formulation dependent.
核心技術：Ultrafiltration & Diafiltration (UF/DF)
技術原理
UF/DF 技術使用尺寸選擇性膜來保留較大分子（蛋白質、核酸、病毒載體、LNP），同時讓較小分子（鹽類、溶劑、緩衝液）通過。
重要性
此技術能在不需手動離心步驟的情況下，實現受控的緩衝液交換、脫鹽與濃縮，保持樣品完整性並確保下游結果的重現性。
常見問題
Unagi Automated Buffer Exchange System - Complete UF/DF Solution
The Unagi automated buffer exchange system represents a breakthrough in ultrafiltration and diafiltration (UF/DF) technology for biopharmaceutical sample preparation. This benchtop platform eliminates the tedious manual steps traditionally required for buffer exchange and concentration, delivering consistent, high-recovery results for proteins, nucleic acids, viral vectors, and lipid nanoparticles.
Parallel Processing with Flexible Volume Range
Process up to 8 samples simultaneously with volume flexibility from 0.5 to 8 mL per sample. This parallel processing capability dramatically increases throughput while maintaining individual sample control. Whether handling precious early-stage discovery samples or larger preparative volumes, Unagi provides the versatility needed across diverse research workflows. The system's modular Una cartridge design allows researchers to run different sample types with varying molecular weight cut-offs in a single batch.
Superior Sample Recovery and Precision
Achieve ≥96% sample recovery during buffer exchange and desalting operations, preserving valuable biomolecules throughout the process. The system offers target concentration accuracy within ±10%, ensuring reproducible results for downstream applications. Available membrane options include regenerated cellulose (10, 30, 100 kDa MWCO) and polyethersulfone (30, 100 kDa MWCO), each selected for minimal protein binding and maximum liquid permeability. This dual-membrane strategy allows researchers to optimize recovery based on specific sample characteristics.
Automated Workflow with Real-Time Monitoring
Unagi's ultrasonic volume sensing technology continuously monitors sample volumes throughout the exchange process, automatically adjusting pressure and mixing parameters to achieve target specifications. The system employs programmable orbital mixing at 700 rpm with adjustable duty cycles, combined with selectable exchange pressures of 15, 30, or 60 psi. This intelligent automation eliminates the need for manual intervention while providing real-time visibility into cycle progress, filtration rates, and percent exchange for each sample. Researchers can pause runs to collect finished samples early or allow the complete batch to process unattended.
Comprehensive Experimental Modes
The platform supports four distinct operational modes: Buffer Exchange for straightforward buffer replacement, Buffer Exchange + Concentrate for simultaneous buffer exchange and volume reduction, Concentrate Only for sample concentration without buffer change, and Reduce Sample Volume for processing dilute samples from up to 48 mL down to 8 mL. This operational flexibility enables researchers to address multiple sample preparation challenges using a single instrument.
Complete Documentation and Compliance
Every Unagi run generates detailed reports documenting final volumes, concentrations, flow rates for each cycle, and achieved exchange percentages. These reports include time stamps, instrument IDs, and experimental parameters, meeting laboratory information management system (LIMS) requirements and supporting regulatory compliance. Export options include experiment logs, summary reports, and raw data files suitable for electronic laboratory notebooks or quality control documentation.
Technical Excellence in Sample Preparation
The system's compact footprint (63 cm W x 57 cm D x 66 cm H, 65 kg) fits standard laboratory benchtops while delivering industrial-grade performance. Operating at room temperature with universal voltage compatibility (100-260 VAC, 50-60 Hz), Unagi requires only compressed nitrogen or dry air (0.55-0.69 MPa, minimum 0.25 L/s flow). The disposable Una cartridges eliminate cleaning validation concerns while ensuring consistent performance. High-precision liquid handling (≤1% dispense accuracy) combined with automatic tip reuse (up to 12 times per exchange) optimizes both accuracy and cost-effectiveness. For protein concentration applications, the system handles concentrations up to 200 mg/mL, making it suitable for formulation development and biophysical characterization workflows. Exchange times for standard antibody samples (96% exchange at 10 mg/mL IgG, 8 mL volume) typically complete within 4.5 hours, though actual times vary with sample characteristics and experimental parameters. This hands-free operation allows laboratory personnel to focus on higher-value activities while Unagi ensures reproducible sample preparation results.