告別手動 Buffer Exchange 的繁瑣流程

Automated Buffer Exchange and Sample Preparation Solutions

Discover Unagi and Big Tuna, revolutionary automated buffer exchange platforms powered by ultrafiltration/diafiltration (UF/DF) technology with intelligent volume tracking. These systems eliminate the tedious, hands-on work traditionally required for buffer exchange and sample concentration in biologics development.

Unagi is the first benchtop buffer exchange platform designed for processing 1-8 samples with volumes ranging from 0.5 to 8 mL. The system uses regenerated cellulose and polyethersulfone membranes with molecular weight cutoffs of 10, 30, and 100 kDa, ensuring ≥96% sample recovery. Unagi continuously monitors sample volumes during runs, automatically calculating and adding the precise amount of new buffer to prevent over-concentration and protein aggregation.

Big Tuna is a fully automated, plate-based buffer exchange system supporting 24-well (0.45-8 mL) or 96-well (100-450 µL) formats. The system provides unprecedented process control through real-time monitoring of individual well volumes and flow rates. This intelligent monitoring prevents fast-flowing samples from drying out while ensuring slower samples complete their exchange cycles. Big Tuna handles protein concentrations from 0.1 to 200 mg/mL, making it ideal for formulation screening, optimization, and process development.

Both systems employ gentle orbital mixing technology, offering significant advantages over traditional dead-end ultrafiltration methods. At high protein concentrations, orbital mixing reduces aggregation risk and prevents gel formation at the membrane surface. This approach maintains faster sample flow and delivers consistent, reproducible results essential for biologics development.

Key applications include antibody formulation screening, recombinant protein buffer exchange, AAV gene therapy vector cleanup and concentration, LNP formulation development, nucleic acid purification, and exosome characterization. The systems excel in pH optimization studies, excipient screening, and high-throughput sample preparation workflows.

Unagi utilizes Una consumables with regenerated cellulose or polyethersulfone membranes, while Big Tuna uses Unfilter plates exclusively with regenerated cellulose membranes to minimize non-specific binding. The Unfilter 96 offers additional flexibility with 3 kDa MWCO option for oligonucleotides and small peptides.

The intelligent volume tracking system represents a breakthrough in preventing protein aggregation during buffer exchange. By continuously monitoring each sample's volume and flow rate, the systems automatically adjust processing parameters to maintain optimal concentration levels throughout the run. This prevents the over-concentration that leads to irreversible protein aggregation and ensures every sample reaches the target exchange percentage and concentration.

From early research to process development, Unagi and Big Tuna streamline workflows and free researchers from time-consuming manual operations. Whether exchanging one protein into eight different buffers or processing 96 samples simultaneously for formulation screening, these automated platforms deliver the consistency and efficiency required for modern biologics development.