MACSima™ 系統｜全自動多重免疫染色與空間生物學平台

The MACSima™ Platform represents a revolutionary breakthrough in spatial biology research, utilizing innovative MICS (MACSima Imaging Cyclic Staining) technology to enable comprehensive multiplex analysis of hundreds of protein and RNA markers on a single tissue section. This fully automated spatial biology platform seamlessly integrates high-quality antibodies, precise sample processing, and sophisticated analysis software to support researchers in flow cytometry extension, multiomics studies, and drug development applications.

At the core of the MACSima system lies the groundbreaking MICS technology, which employs a unique three-step iterative process: stain, image, and erase. This innovative approach captures microscopy data of hundreds of markers without compromising sample integrity for downstream processing. The platform utilizes pretested recombinant antibody-fluorochrome conjugates from Miltenyi Biotec, providing reliable and reproducible staining results. The automated widefield microscope system acquires high-resolution epifluorescence images with exceptional quality, while the proprietary signal erasure mechanism ensures complete removal of fluorescent signals between cycles.

The MACSima Platform offers unparalleled flexibility in spatial biology research, supporting analysis of various fixed sample types including formalin-fixed paraffin-embedded (FFPE) tissues, frozen sections (PFA or acetone-fixed), adherent cells, and suspension cells. The specialized MACSwell sample carrier system provides optimized reaction cavities for different sample formats, ensuring trouble-free MICS experiments across diverse research applications.

Complementing the hardware excellence is the intuitive MACS iQ View - Spatial Biology software package, specifically designed to handle and analyze the vast amounts of valuable data generated by the MACSima Platform. The software features interactive and dynamic data display capabilities, enabling researchers to visualize experimental results through various graphs, tables, and plotting options. Advanced analysis tools include dimension reduction plots (t-SNE and UMAP), distance mapping, heatmaps, and sophisticated gating and clustering options for deep phenotyping analysis.

The platform's applications span multiple research areas, including deep phenotyping for understanding cell heterogeneity and microenvironment interactions, biomarker research and discovery for patient stratification strategies, and drug target discovery and validation in preclinical studies. Researchers worldwide have utilized the MACSima Platform to generate groundbreaking discoveries, from creating comprehensive cellular atlases of human organs to identifying novel therapeutic targets for cancer treatment.

With its integration of RNAsky™ technology, the MACSima Platform enables same-section spatial multiomics analysis, combining ultrahigh-plex spatial proteomics with RNA transcript detection on the same tissue section. This capability provides researchers with unprecedented insights into the complex molecular landscape of tissues, advancing our understanding of disease mechanisms and therapeutic interventions. The platform's automated operation, extensive storage capacity, and robust performance specifications make it an ideal solution for academic institutions and pharmaceutical companies seeking to advance their spatial biology research capabilities.